Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
Autor: | M. Raica, A.M. Cimpean, Ş.F. Avram |
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Rok vydání: | 2013 |
Předmět: |
Pathology
medicine.medical_specialty animal structures Chick embryo chorioallantoic membrane Physiology Angiogenesis Immunology Biophysics chemical and pharmacologic phenomena Ocean Engineering Chick Embryo Biology Biochemistry Chorioallantoic Membrane Immunophenotyping In vivo Cell Line Tumor medicine Animals General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 lcsh:R5-920 P1.HTR Neovascularization Pathologic General Neuroscience Biomedical Sciences Embryo Mastocytoma Cell Biology General Medicine medicine.disease Mast cell Immunohistochemistry Cell biology Chorioallantoic membrane medicine.anatomical_structure lcsh:Biology (General) Cell culture embryonic structures lcsh:Medicine (General) |
Zdroj: | Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 1, Pages: 52-57, Published: 11 JAN 2013 Brazilian Journal of Medical and Biological Research, Vol 46, Iss 1, Pp 52-57 (2013) Brazilian Journal of Medical and Biological Research v.46 n.1 2013 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
ISSN: | 1414-431X |
Popis: | The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs. |
Databáze: | OpenAIRE |
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