Inhibition of viral assembly in murine cells by HIV-1 matrix

Autor: Wolfgang Hübner, Benjamin K. Chen
Rok vydání: 2006
Předmět:
HIV Antigens
viruses
Human immunodeficiency virus (HIV)
Matrix (biology)
medicine.disease_cause
gag Gene Products
Human Immunodeficiency Virus
Mice
Retrovirus
HIV Antigens/chemistry
Murine leukemia virus
gag/physiology
Viral
biology
Viral Proteins/chemistry
Leukemia Virus
3T3 Cells
Phenotype
Cell biology
Leukemia Virus
Murine
gag/chemistry
Membrane
Murine/genetics
Viral Proteins/genetics
gag/genetics
Human Immunodeficiency Virus
Gene Expression Regulation
Viral
HIV-1/genetics
Gene Products
gag
Virus
Cell Line
Viral Assembly
Viral Matrix Proteins
Viral Proteins
Viral Matrix Proteins/physiology
Virology
HIV Antigens/genetics
medicine
Humans
Gene Products
Animals
Virus Assembly/drug effects
gag Gene Products
HIV-1/metabolism
Chimera
Virus Assembly
Cell Membrane/metabolism
Cell Membrane
Murine/metabolism
HIV-1/chemistry
biology.organism_classification
Viral Proteins/physiology
Gene Expression Regulation
HIV-1
HIV Antigens/physiology
Gene Deletion
Cell Membrane/virology
HIV-1/pathogenicity
Zdroj: Virology. 352:27-38
ISSN: 0042-6822
DOI: 10.1016/j.virol.2006.04.024
Popis: In human cells, the N-terminal matrix (MA) domain of the human immunodeficiency virus type 1 (HIV-1) Gag targets assembly to specific membrane compartments. In murine fibroblasts, membrane targeting of Gag and assembly of HIV-1 are inefficient. These deficiencies are relieved by replacement of HIV-1 MA with murine leukemia virus (MLV) MA in chimeric proviruses. In this study, we examined chimeric HIV-1 carrying tandem MLV and HIV-1 MA domains and found that the addition of MLV MA to the N-terminus of HIV-1 Gag enhanced membrane binding in murine cells, but was not sufficient to stimulate virus production. Removal of HIV MA was required to observe more efficient Gag processing and increased virus production in murine cells. Deletion of the globular head of MA also alleviated the blocks to membrane binding and Gag processing in murine cells, yet did not lead to increased virus production. These MA-dependent, cell-type-specific phenotypes suggest that host factors interact with the globular head of MA to regulate membrane binding and additional membrane-independent step(s) required for assembly.
Databáze: OpenAIRE
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