Knockdown of the DUF647 family member RUS4 impairs stamen development and pollen maturation in Arabidopsis
| Autor: | Wen-Chao Li, Ya-Jie Chen, Shu-Qing Zhao, Xiao-Xue Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Stamen Cyclopentanes Flowers Plant Science medicine.disease_cause 01 natural sciences 03 medical and health sciences Plant Growth Regulators Microspore Gene Expression Regulation Plant Pollen Genetics medicine Arabidopsis thaliana MYB Oxylipins Pollen maturation biology Arabidopsis Proteins Gene Expression Regulation Developmental Membrane Proteins food and beverages General Medicine Anther dehiscence biology.organism_classification Cell biology 030104 developmental biology Gene Knockdown Techniques Agronomy and Crop Science Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Science. 301:110645 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2020.110645 |
| Popis: | ROOT UV-B SENSITIVE4 (RUS4) encodes a Domain of Unknown Function647 (DUF647) protein, whose function is poorly understood. We have previously shown the artificial microRNA knockdown Arabidopsis RUS4 plants, referred to as amiR-RUS4, have severely reduced male fertility with a defect in anther dehiscence. Here, we show that amiR-RUS4 plants are also defective in pollen maturation and germination. Promoter-reporter analysis shows that RUS4 is highly expressed in tapetal layer, developing microspores, mature and germinating pollen, strongly suggesting its role in the process of pollen maturation. As the translational RUS4-GFP fusion protein has been localized to the chloroplasts where the first step of jasmonic acid (JA) biosynthesis takes place, leading to the hypothesis that RUS4 may be involved in JA-mediated stamen development. We show that expression of several JA metabolic genes increased markedly in flower buds of the amiR-RUS4 plants compared to that of the wild-type. We further show that transcript abundance of a clade of the JA-responsive MYB transcript factor genes, especially MYB108, reduced significantly in stamens of amiR-RUS4 plants relative to the wild-type; these MYB transcript factors have been shown to be required for JA-mediated stamen and pollen maturation. Our data suggest that RUS4 may play a role in coordinating anther dehiscence and pollen maturation by affecting the expression of JA-related genes. |
| Databáze: | OpenAIRE |
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