Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats
| Autor: | Horea Benea, Gabriela Dogaru, Alexandru Mester, Mihai Gheorghe Paiusan, Dragos Apostu, Catalin Popa, Gyorgy Istvan Bodizs, Gheorghe Tomoaia, Elena Mihaela Jianu, Alina Mihaela Toader, Cristian Berce, Daniel Oltean-Dan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Osteocalcin Enzyme-Linked Immunosorbent Assay Bone healing New and Emerging Methods Bone and Bones Bone remodeling 03 medical and health sciences 0302 clinical medicine Calcification Physiologic Electromagnetic Fields Osteogenesis medicine Animals Postoperative Period Rats Wistar Fracture Healing Femur fracture osteogenic markers lcsh:R5-920 Osteoblasts biology business.industry Fibrocartilage General Medicine X-Ray Microtomography bone consolidation medicine.disease Rats titanium 6-aluminum-4-vanadium 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Callus biology.protein Alkaline phosphatase Bone marrow Nuclear medicine business lcsh:Medicine (General) Femoral Fractures bone remodelling Calcification |
| Zdroj: | Bosnian Journal of Basic Medical Sciences (2019) |
| ISSN: | 1840-4812 |
| Popis: | In vitro studies showed that high-frequency pulsed electromagnetic fields (HF-PEMFs) increase the activity/expression of early and late osteogenic markers and enhance bone mineralization. The main aim of this study was to investigate the in vivo effects of HF-PEMFs on fracture healing using a rat model. A femur fracture was established by surgery in 20 male Wistar rats. Titanium nails were implanted to reduce and stabilize the fracture. After surgery, 20 rats were equally divided into untreated control and treated group (from the first postoperative day HF-PEMFs at 400 pulses/sec [pps] were applied for 10 minutes/day, for two weeks). Quantitative and qualitative assessment of bone formation was made at two and eight weeks following surgery and included morphological and histological analysis, serological analysis by ELISA, micro-computed tomography (micro-CT), and three-point bending test. At two weeks in HF-PEMF group, soft callus was at a more advanced fibrocartilaginous stage and the bone volume/total tissue volume (BV/TV) ratio in the callus area was significantly higher compared to control group (p = 0.047). Serum concentration of alkaline phosphatase (ALP) and osteocalcin (OC) was significantly higher in HF-PEMF group (ALP p = 0.026, OC p = 0.006) as well as the mechanical strength of femurs (p = 0.03). At eight weeks, femurs from HF-PEMF group had a completely formed woven bone with dense trabeculae, active bone marrow, and had a significantly higher BV/TV ratio compared to control (p = 0.01). HF-PEMFs applied from the first postoperative day, 10 minutes/day for two weeks, enhance bone consolidation in rats, especially in the early phase of fracture healing. |
| Databáze: | OpenAIRE |
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