Heparan Sulfate Mimetics Accelerate Postinjury Skeletal Muscle Regeneration
| Autor: | Jessica Bouvière, Sébastien Banzet, Aurélie Trignol, Dieu-Huong Hoang, Bénédicte Chazaud, Peggy Del Carmine, Denis Barritault, Marie-Emmanuelle Goriot, Sabrina Ben Larbi |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Angiogenesis 0206 medical engineering Biomedical Engineering Neovascularization Physiologic Bioengineering Context (language use) Stimulation 02 engineering and technology Muscle Development Biochemistry Cell Fusion Biomaterials Extracellular matrix 03 medical and health sciences Biomimetic Materials medicine Animals Regeneration Muscle Skeletal 030304 developmental biology Muscle Cells 0303 health sciences Myogenesis business.industry Regeneration (biology) Endothelial Cells Skeletal muscle 020601 biomedical engineering Cell biology Mice Inbred C57BL medicine.anatomical_structure Heparitin Sulfate Stem cell business |
| Zdroj: | Tissue Engineering Part A. 25:1667-1676 |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Although skeletal muscle is capable of complete recovery after an injury, specific situations require support or acceleration of this process, such as in the elderly and athletes, respectively. Skeletal muscle regeneration is due to muscle stem cells (MuSCs) that undergo adult myogenesis, a process sustained by MuSC environment. Although recognized as important, extracellular matrix (ECM) has been overlooked in this process. Matrix-based therapy aims at improving ECM remodeling to support tissue repair. In this context, we investigated the properties of a single injection of the clinical grade glycosaminoglycan mimetics RGTA® (ReGeneraTing Agents) on skeletal muscle regeneration in a context compatible with a clinical application, that is, 3 days after the injury. Our results show that RGTA-treated muscles showed an increase of the number of myonuclei in regenerating myofibers and an increase of the capillarization of the new myofibers. In vitro experiments showed that RGTA directly acts on MuSCs by stimulating their fusion into myotubes and on endothelial cells by stimulating the formation and maturation of vessels in a 3D culture setup. These results indicate that a single administration of RGTA in regenerating muscle stimulated both myogenesis and angiogenesis, thus accelerating skeletal muscle regeneration. Impact Statement Although highly powerful in normal condition, postinjury skeletal muscle regeneration is less efficient in some situations, such as obese, elderly, or resting people. In other context, such as high-performance sport, skeletal muscle regeneration must be shortened but in a way ensuring a full functional recovery. In this context, our results show that a single injection of the clinical grade glycosaminoglycan mimetics RGTA® (ReGeneraTing Agents), in a context compatible with a clinical application, that is, 3 days after the injury, is beneficial for skeletal muscle regeneration, through the stimulation of both myogenesis and angiogenesis. |
| Databáze: | OpenAIRE |
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