Human embryonic stem cell dispersion in electrospun PCL fiber scaffolds by coating with laminin-521 and E-cadherin-Fc
| Autor: | Carolina Åstrand, Robert McKean, Veronique Chotteau, Mattias Leino, Nanayaa F. Hughes-Brittain, Brendan Robb |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Cellular differentiation Polyesters Cell- och molekylärbiologi Cell Population Human Embryonic Stem Cells Biomedical Engineering Nanofibers Gene Expression Biomaterials Extracellular matrix 03 medical and health sciences electrospun scaffold three-dimensional culture extracellular matrix coating medicine Humans Myocytes Cardiac Progenitor cell education Induced pluripotent stem cell Cells Cultured Neurons education.field_of_study Tissue Scaffolds Cell Differentiation Cadherins Embryonic stem cell Cell biology Extracellular Matrix 030104 developmental biology medicine.anatomical_structure PCL Hepatocytes RNA Laminin Stem cell pluripotent stem cells Cell and Molecular Biology Biomedical engineering |
| Popis: | Advances in human pluripotent cell cultivation and differentiation protocols have led to production of stem cell-derived progenitors as a promising cell source for replacement therapy. Three-dimensional (3-D) culture is a better mimic of the natural niche for stem cells and is widely used for disease modeling. Here, we describe a nonaggregate culture system of human embryonic stem cells inside electrospun polycaprolactone (PCL) fiber scaffolds combined with defined extracellular proteins naturally occurring in the stem cell niche. PCL fiber scaffolds coated with recombinant human laminin-521 readily supported initial stem cell attachment and growth from a single-cell suspension. The combination of recombinant E-cadherin-Fc and laminin-521 further improved cell dispersion rendering a uniform cell population. Finally, we showed that the cells cultured in E-cadherin-Fc- and laminin-521-coated PCL scaffolds could differentiate into all three germ layers. Importantly, we provided a chemically defined 3-D system in which pluripotent stem cells grown and differentiated avoiding the formation of cell aggregates. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1226-1236, 2018. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|