A flow cytometric assay for simultaneous assessment of drug efflux, proliferation, and apoptosis
| Autor: | K. A. Muirhead, Jean Boutonnat, M. Barbier, B. D. Gray, Xavier Ronot |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cell division
Biophysics Apoptosis Drug resistance Biology Irinotecan Pathology and Forensic Medicine Endocrinology Predictive Value of Tests Humans Fluorescent Dyes Staining and Labeling Cell Biology Hematology Cell cycle Flow Cytometry Phenotype Molecular biology Antineoplastic Agents Phytogenic Staining Multiple drug resistance Drug Resistance Neoplasm Camptothecin Efflux K562 Cells Cell Division |
| Zdroj: | Cytometry. Part B, Clinical cytometry. 59(1) |
| ISSN: | 1552-4949 |
| Popis: | Background Proliferative status and multidrug resistance play a key role in determining cell response to chemotherapy. There is a need to develop multiple labeling method that allows simultaneous assessment of multidrug resistant (MDR) phenotype, proliferative status, apoptosis related changes in mitochondrial potential, in chemosensitive and chemoresistant tumor cell populations. Methods A three-color labeling was performed using Hoechst 33342 (DNA), JC1 (mitochondrial potential), and a far red fluorescent membrane intercalating dye: PTIR271 (proliferation). Results Combined staining of DNA and mitochondrial potential allows identification of subpopulations expressing and MDR phenotype mediated by P-glycoprotein (Pgp), and, in Pgp negative subpopulations, identification of apoptotic cells and evaluation of cell cycle status in viable cells. Addition of a far red fluorescent membrane intercalating dye, PTIR271, allows simultaneous monitoring of cell division status by dye dilution in both drug sensitive and drug resistant populations. Conclusion This triple labeling is an interesting method to study the proliferation status of drug sensitivity and drug resistance in viable tumor cells. © 2004 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text