Decrease of intracellular fluorescein fluorescence polarization (IFFP) in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA), concanavalin A (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody
| Autor: | Motti Deutsch, Shoshana Baron, Beatriz Lifschitz-Mercer, Oleg Marder, Dalia Dotan, Reuven Tirosh, Arye Weinreb, Avi Eisenthal, Samario Chaitchik |
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| Rok vydání: | 1996 |
| Předmět: |
CD3 Complex
Lymphocyte CD3 Population Fluorescence Polarization chemical and pharmacologic phenomena Lymphocyte Activation chemistry.chemical_compound Antibody Specificity Lectins Concanavalin A medicine Humans Lymphocytes Phytohemagglutinins Fluorescein education Phytohaemagglutinin education.field_of_study Dose-Response Relationship Drug biology Pokeweed mitogen Lectin Cell Biology General Medicine Fluoresceins Molecular biology Kinetics medicine.anatomical_structure Pokeweed Mitogens chemistry Microscopy Electron Scanning biology.protein |
| Zdroj: | Biology of the Cell. 86:145-150 |
| ISSN: | 0248-4900 |
| Popis: | In the present study we describe the induction of changes in intracellular fluorescein fluorescence polarization (IFFP) in lymphocytes undergoing activation with a variety of stimulants. These stimulants included the lectins phytohaemagglutinin (PHA), concanavalin (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody. Changes in IFFP were detected in individual cells using the Cellscan apparatus. Our results show that by employing mitogenic concentrations of PHA, as revealed in a [3H]-thymidine incorporation assay, a decrease in the IFFP in human peripheral blood lymphocytes (PBL) occurred within 40 min. ConA and anti-CD3 affected similarly IFFP, whereas PWM, a B lymphocyte lectin, had no effect on IFFP at the concentrations employed. Kinetic analysis revealed that changes in IFFP occurred within 20-40 min after exposure to the stimulants and lasted for 24 h. Our results show that stimulants which activate CD3+ lymphocytes caused immediate changes in IFFP, in an enriched population of human PBL. The possible mechanisms involved in IFFP modulation following exposure to selected stimulants are discussed. |
| Databáze: | OpenAIRE |
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