Joint genome-wide association and transcriptome sequencing reveals a complex polygenic network underlying hypocotyl elongation in rapeseed (Brassica napus L.)
| Autor: | Bin Yi, Shengwei Dou, Kaining Hu, Ziru Zeng, Tingdong Fu, Jinxiong Shen, Zhifei Xue, Xiang Luo, Jinxing Tu, Chaozhi Ma |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Population Quantitative Trait Loci Single-nucleotide polymorphism Genome-wide association study Polymorphism Single Nucleotide Linkage Disequilibrium Article Hypocotyl Transcriptome 03 medical and health sciences Quantitative Trait Heritable Gene Regulatory Networks education Gene Genetics education.field_of_study Multidisciplinary biology Gene Expression Profiling fungi Brassica napus food and beverages Chromosome Mapping Genetic Variation High-Throughput Nucleotide Sequencing biology.organism_classification 030104 developmental biology Genetics Population Phenotype Gene Expression Regulation Haplotypes Seedling Seedlings Doubled haploidy Genome-Wide Association Study |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Hypocotyl elongation is considered an important typical seedling trait contributing directly to an increase in and stabilization of the yield in Brassica napus, but its molecular genetic mechanism is poorly understood. In the present study, hypocotyl lengths of 210 lines were measured in an illuminated culture room. A genome-wide association study (GWAS) was performed with 23,435 single nucleotide polymorphisms (SNPs) for hypocotyl length. Three lines with long hypocotyl length and three lines with short hypocotyl length from one doubled haploid line (DH) population were used for transcriptome sequencing. A GWAS followed by transcriptome analysis identified 29 differentially expressed genes associated with significant SNPs in B. napus. These genes regulate hypocotyl elongation by mediating flowering morphogenesis, circadian clock, hormone biosynthesis, or important metabolic signaling pathways. Among these genes, BnaC07g46770D negatively regulates hypocotyl elongation directly, as well as flowering time. Our results indicate that a joint GWAS and transcriptome analysis has significant potential for identifying the genes responsible for hypocotyl elongation; The extension of hypocotyl is a complex biological process regulated by a polygenic network. |
| Databáze: | OpenAIRE |
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