Fucan effect on CHO cell proliferation and migration
| Autor: | Hugo Alexandre Oliveira Rocha, Ruth Medeiros Oliveira, Valquíria P. Medeiros, Helena B. Nader, Jailma Almeida-Lima, Célia Regina Cavichiolo Franco, Arthur Anthunes Jacome Vidal, Leonardo Thiago Duarte Barreto Nobre, Edvaldo S. Trindade, Edgar J. Paredes-Gamero |
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| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
Polymers and Plastics Integrin Antineoplastic Agents CHO Cells Flow cytometry chemistry.chemical_compound Cricetulus Cell Movement Polysaccharides Fucoidan Cricetinae Drug Discovery medicine Materials Chemistry Animals Cancer Cell Proliferation Fucose medicine.diagnostic_test biology Cell growth Cell Cycle Organic Chemistry MAPK pathway Cell cycle Cell biology Fibronectins Fibronectin Enzyme Activation chemistry biology.protein Antiproliferative Mitogen-Activated Protein Kinases G1 phase |
| Zdroj: | Carbohydrate Polymers. 98(1):224-232 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2013.05.040 |
| Popis: | Fucan is a term used to denominate sulfated l-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schröederi seaweed. This 21.5kDa galactofucan inhibited CHO-K1 proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs. |
| Databáze: | OpenAIRE |
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