Effect of diet and nonesterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows
| Autor: | Cinzia Marchitelli, Zhangrui Cheng, D Claire Wathes, Alessandra Crisà, Tine Rousing, Klaus Lønne Ingvartsen, Leslie Foldager |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Biological adhesion Ice calving nonesterified fatty acids Fatty Acids Nonesterified Biology Peripheral blood mononuclear cell 03 medical and health sciences cows NEFA Immune system Internal medicine Lactation Gene expression Genetics medicine Animals 030304 developmental biology 2. Zero hunger chemistry.chemical_classification 0303 health sciences 3-Hydroxybutyric Acid Postpartum Period 0402 animal and dairy science Fatty acid RNA sequencing 04 agricultural and veterinary sciences peripheral blood mononuclear cells 040201 dairy & animal science Diet Milk Endocrinology medicine.anatomical_structure chemistry Leukocytes Mononuclear Cattle Female Animal Science and Zoology Transcriptome Food Science |
| Zdroj: | Cheng, Z, Wylie, A, Ferris, C, Ingvartsen, K L, Wathes, D C & GplusE Consortium 2021, ' Effect of diet and nonesterified fatty acid levels on global transcriptomic profiles in circulating peripheral blood mononuclear cells in early lactation dairy cows ', Journal of Dairy Science, vol. 104, no. 9, pp. 10059-10075 . https://doi.org/10.3168/jds.2021-20136 |
| Popis: | After calving, lipid mobilization caused by increased nutrient demands for lactation leads to elevated circulating concentrations of nonesterified fatty acids (NEFA). Excessive NEFA levels have previously been identified as a major risk factor for postpartum immunosuppression. The aim of this study was to investigate changes in global transcriptomic gene expression of peripheral blood mononuclear cells (PBMC) in dairy cows offered different early lactation diets (high concentrate, n = 7; medium, n = 8; or low, n = 9) and with differing circulating levels of NEFA. Cows were classified as having NEFA concentrations of either 750 µM (high, n = 10) at 14 d in milk. Plasma urea concentrations were greater for cows on the high concentrate diet but β-hydroxybutyrate and glucose concentrations did not differ significantly between either dietary treatments or NEFA groups. Cows with high NEFA weighed more at drying off and suffered greater body condition score loss after calving. The PBMC were isolated at 14 d in milk, and RNA was extracted for RNA sequencing. Differential gene expression was analyzed with DESeq2 with q-value for false discovery rate control followed by Gene Ontology Enrichment. Although there were no differentially expressed genes associated with lactation diet, 304 differentially expressed genes were identified between cows with high and low circulating NEFA, with 118 upregulated and 186 downregulated. Gene Ontology enrichment analysis demonstrated that biological adhesion and immune system process were foremost among various PBMC functions which were altered relating to body defenses and immunity. High NEFA concentrations were associated with inhibited cellular adhesion function by downregulating 20 out of 26 genes (by up to 17-fold) related to this process. Medium NEFA concentrations altered a similar set of functions as high NEFA, but with smaller enrichment scores. Localization and immune system process were most significant, with biological adhesion ranking only eleventh. Our results demonstrated that increased circulating NEFA concentrations, but not diet, were associated with immune system processes in PBMC in early lactation cows. Leukocyte cell-to-cell adhesion was inhibited when the NEFA concentration exceeded 750 µM, which would reduce the efficiency of diapedesis and so contribute to decreased body defense mechanisms and predispose animals to infection. |
| Databáze: | OpenAIRE |
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