Peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects
| Autor: | Robert A. Sikes, Gina Beck, Joshua R. Bush, Haixiang Liang, Quanjun Cui, Vedavathi Madhu, Gary Balian |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Bone sialoprotein
Male Bone Regeneration General Chemical Engineering Gene Expression Bone healing Matrix (biology) General Biochemistry Genetics and Molecular Biology Mice Osteogenesis Peptide Library Animals Peptide library Bone regeneration Mice Inbred BALB C biology General Immunology and Microbiology Chemistry General Neuroscience Mesenchymal stem cell Mesenchymal Stem Cells Molecular biology Rats Inbred F344 Rats Cellular Biology biology.protein Osteocalcin Alkaline phosphatase Bone Diseases Peptides |
| Zdroj: | Journal of visualized experiments : JoVE. (46) |
| ISSN: | 1940-087X |
| Popis: | Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects on bone repair in a unicortical defect model. Treatment of mesenchymal cells produced an increase in alkaline phosphatase activity, showed nodule formation by the cells, and increased the expression of genes for runx2, osterix, bone sialoprotein, and osteocalcin. A collagen sponge soaked with peptide promoted repair of bone defects, whereas the control was less effective. The results from this study demonstrated that mesenchymal cells treated with peptide in vitro differentiate towards osteogenesis, and, that peptides delivered in vivo using a collagen sponge promote the repair of unicortical defects. |
| Databáze: | OpenAIRE |
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