Normal serum increases adhesion of neutrophils to tracheal epithelial cells by a CD11b/CD18-dependent mechanism
| Autor: | Netta Joseph-Lerner, Inna Frolkis, Shabtai Varsano, Tamar Reshef |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Hot Temperature Cations Divalent Neutrophils Neutrophile Clinical Biochemistry Radioimmunoassay Biology Dogs Cell–cell interaction Antigens CD Cell Adhesion medicine Animals Humans Cell adhesion Molecular Biology Cells Cultured Tracheal Epithelium CD11 Antigens Cell adhesion molecule Antibodies Monoclonal Blood Proteins Cell Biology Adhesion Immunohistochemistry Molecular biology Blood proteins Epithelium Molecular Weight Trachea medicine.anatomical_structure CD18 Antigens Complement C3b Immunology Female |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 10:298-305 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.10.3.7906949 |
| Popis: | Airway inflammation is characterized by intraluminal influx of inflammatory cells, exudation of plasma, and increased procoagulant activity. We speculated that inflammatory cells might adhere to the airway surface epithelium in order to better localize and regulate airway inflammatory responses. Therefore, in this study, we asked whether neutrophils adhere to airway epithelial cells, whether serum or plasma factors increase adhesion, and, if so, what the characteristics of the involved adhesion molecules are. To answer these questions, we incubated human 51Cr-labeled neutrophils from peripheral blood with dog tracheal epithelial cells in culture in the presence or absence of normal human serum or plasma. After 30 min, nonadhering neutrophils were centrifuged away and neutrophil adhesion was assessed by radioassay. We found that unstimulated adhesion of neutrophils to cultured epithelial cells was quite low (6%). However, incubation with 10% serum or plasma increased adhesion of neutrophils to epithelial cells dramatically (up to a mean of 71%). The serum-induced increase in adhesion was concentration dependent; even 1% serum was effective (19% adhesion). Serum adhesion factor acted selectively on epithelial surfaces, was heat sensitive, had a molecular weight12,000, and depended on the presence of divalent cations. mAb 60.3 (anti-CD18) and mAb anti-Mol (anti-CD11b, anti-CR3) inhibited serum-induced adhesion by50% each. We conclude that normal serum and plasma contain a potent adhesion factor that induces adhesion of neutrophils to tracheal epithelium in culture.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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