Establishment and characterization of a new endometrial cancer cell line (SCRC-1)
| Autor: | Roger L. Ladda, Justine Gorodecki, Fred Rapp, Laszlo Geder, Rodrigue Mortel, Samuel P. Ward |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Cytomegalovirus Mice Nude Adenocarcinoma Biology Immunofluorescence Epithelium Cell Line Mice Multinucleate Antigens Neoplasm medicine Animals Humans Simplexvirus Trypsin Antigens Viral medicine.diagnostic_test Endometrial cancer Obstetrics and Gynecology Fibroblasts Middle Aged medicine.disease Molecular biology High Mitotic Activity Kinetics medicine.anatomical_structure Cell culture Cytoplasm Karyotyping Uterine Neoplasms Female Ploidy |
| Zdroj: | American Journal of Obstetrics and Gynecology. 135:671-679 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/s0002-9378(16)32994-5 |
| Popis: | A new human endometrial cell line, SCRC-1, has been established from a uterine adenocarcinoma. During a period of 57 weeks in culture, the SCRC-1 cells have been passaged over 100 times. Cultures have an epithelioid morphology, high mitotic activity, and a tendency to form multiple cell layers. The cells are polygonal and are arranged in pavement-like fashion. Most have multiple nucleoli, some are multinucleated, and all have heavily vacuolated cytoplasm. Pretreatment with iododeoxyuridine did not reveal viral markers by cocultivation or immunofluorescence techniques. Karyologic studies indicate that the SCRC-1 cell line is essentially diploid with a small subpopulation of tetraploid cells. Over a period of 20 weeks, 1 of 14 athymic nude mice inoculated with SCRC-1 cells developed a tumor which histologically closely resembled the original human tumor. Cells were subsequently propagated from this tumor and the resulting epithelioid-like cell line was designated SCRC-1, Tu 1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|