Collagen Hydrogel Functionalized with Collagen-Targeting IFNA2b Shows Apoptotic Activity in Nude Mice with Xenografted Tumors
| Autor: | Jin-Kui Pi, Jesse Li-Ling, Jun-Gen Hu, Xiao-Fan Liu, Huiqi Xie, Yan-Lin Jiang |
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| Rok vydání: | 2018 |
| Předmět: |
Chemistry
medicine.medical_treatment Biomedical Engineering Alpha interferon 02 engineering and technology Matrix (biology) 021001 nanoscience & nanotechnology Fusion protein In vitro Cell biology Biomaterials 03 medical and health sciences 0302 clinical medicine Cytokine Tissue engineering In vivo 030220 oncology & carcinogenesis Self-healing hydrogels medicine 0210 nano-technology |
| Zdroj: | ACS Biomaterials Science & Engineering. 5:272-282 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.8b00490 |
| Popis: | Interferon alpha 2b (IFNA2b) has been used in immunotherapy for cancers with certain success. To reduce fast diffusion of IFNA2b and consequent dose-dependent side effects, we constructed a collagen hydrogel loaded with IFNA2b fused to collagen-binding domain by using methods of tissue engineering. The fusion protein showed apoptotic activity similar to that of native IFNA2b against MCF-7 cells in vitro, but with relatively higher affinity for collagen type I. Accordingly, the former diffused out of the collagen matrix slower than the latter. Importantly, collagen hydrogels loaded with the fusion protein possessed apoptotic activity in vitro and released the engineered cytokine in a controlled manner. In addition, such hydrogels reduced tumor size and extended the survival of the mouse model with xenografted tumors, which suggested a moderate antitumor activity in vivo. |
| Databáze: | OpenAIRE |
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