Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death
| Autor: | Berend Snijder, Eric D Lee, Georg E. Winter, Brent R. Stockwell, Leila S. Musavi, Giulio Superti-Furga, Manuele Rebsamen, Scott J. Dixon |
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| Rok vydání: | 2015 |
| Předmět: |
Programmed cell death
Apoptosis Biology Haploidy Biochemistry ACSL4 Insertional mutagenesis Small Molecule Libraries chemistry.chemical_compound Cell Line Tumor Humans Letters Gene Genetics Regulation of gene expression Fatty acid metabolism Lipid metabolism General Medicine Lipid Metabolism 3. Good health Cell biology Mutagenesis Insertional chemistry Gene Expression Regulation Cell culture Molecular Medicine |
| Zdroj: | ACS Chemical Biology ACS chemical biology |
| ISSN: | 1554-8937 |
| Popis: | Little is known about the regulation of nonapoptotic cell death. Using massive insertional mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule-induced nonapoptotic cell death, including mediators of fatty acid metabolism (ACSL4) and lipid remodeling (LPCAT3) in ferroptosis. One novel compound, CIL56, triggered cell death dependent upon the rate-limiting de novo lipid synthetic enzyme ACC1. These results provide insight into the genetic regulation of cell death and highlight the central role of lipid metabolism in nonapoptotic cell death. |
| Databáze: | OpenAIRE |
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