Effect of phosphatidylcholine–cholesterol liposomes on Entamoeba histolytica virulence
| Autor: | Jesús Serrano-LunaJ. Serrano-Luna, Manuel Gutiérrez-MezaM. Gutiérrez-Meza, Víctor TsutsumiV. Tsutsumi, Ricardo Mejía-Zepeda, Silvia Galindo-Gómez, Mineko Shibayama |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Erythrocytes Virulence Factors Immunology Virulence Lobosea Applied Microbiology and Biotechnology Microbiology Entamoeba histolytica chemistry.chemical_compound Phagocytosis Cricetinae Phosphatidylcholine parasitic diseases Concanavalin A Genetics Animals Trophozoites Axenic Molecular Biology Liposome biology General Medicine biology.organism_classification Endocytosis In vitro Culture Media Cholesterol chemistry Liposomes Liver Abscess Amebic Phosphatidylcholines Protozoa lipids (amino acids peptides and proteins) Peptide Hydrolases |
| Zdroj: | Canadian Journal of Microbiology. 56:987-995 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w10-088 |
| Popis: | Trophozoites of Entamoeba histolytica HM-1:IMSS become less virulent after long-term maintenance in axenic cultures. The factors responsible for the loss of virulence during in vitro cultivation remain unclear. However, it is known that in vitro cultivation of amoeba in culture medium supplemented with cholesterol restores their virulence. In this study, we analyzed the effect of adding phosphatidylcholine–cholesterol (PC–Chol) liposomes to the culture medium and evaluated the effect of this lipid on various biochemical and biological functions of E. histolytica HM-1:IMSS in terms of its virulence. The addition of PC–Chol liposomes to the culture medium maintained the virulence of these parasites against hamster liver at the same level as the original virulent E. histolytica strain, even though these amoebae were maintained without passage through hamster liver for 18 months. The trophozoites also showed increased endocytosis, erythrophagocytosis, and carbohydrate residue expression on the amoebic surface. Protease activities were also modified by the presence of cholesterol in the culture medium. These findings indicate the capacity of cholesterol to preserve amoeba virulence and provide an alternative method for the maintenance of virulent E. histolytica trophozoites without the need for in vivo procedures. |
| Databáze: | OpenAIRE |
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