Effect of treatment interruption monitored by CD4 cell count on mitochondrial DNA content in HIV-infected patients: a prospective study
| Autor: | Andrea Cossarizza, Andrea Bedini, Elisa Nemes, Leonarda Troiano, Roberto Esposito, Vanni Borghi, Roberto Bugarini, Milena Nasi, Cristina Mussini, Giovanni Guaraldi, Caroline A. Sabin, Marcello Pinti |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Mitochondrial DNA HAART Time Factors Anti-HIV Agents CD8 cell count medicine.medical_treatment Immunology CD4 cell count HIV Infections CD8-Positive T-Lymphocytes Biology DNA Mitochondrial Polymerase Chain Reaction Drug Administration Schedule law.invention Andrology law Antiretroviral Therapy Highly Active medicine Humans Immunology and Allergy Cytotoxic T cell Prospective Studies Polymerase chain reaction Chemotherapy treatment interruption mitochondial DNA Middle Aged Viral Load CD4 Lymphocyte Count Mitochondria Discontinuation Infectious Diseases Toxicity Drug Evaluation Reverse Transcriptase Inhibitors Female Viral load CD8 |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/01.aids.0000186019.47297.0d |
| Popis: | Background: HIV infection per se and HAART can alter mitochondrial functionality, leading to a decrease in mitochondrial DNA content.Objective: To evaluate whether treatment interruption monitored by CD4 cell count can restore mitochondrial DNA content in peripheral blood lymphocytes.Methods: Mitochondrial DNA content was measured in platelet-free CD4 and CD8 T cells by real-time polymerase chain reaction; flow cytometry was used to identify and quantify activated CD4 and CD8 T lymphocytes.Results: The 30 patients had been treated for a mean of 107 months (range, 27-197). Median CD4 cell count at discontinuation was 702 cells/mu l (range, 547-798). Median observational time from HAART discontinuation was 11.3 months (range, 4-26). Discontinuation of treatment provoked significant increases in mitochondrial DNA in CD8 T cells, which started only 6 months after therapy cliscontinuation [5.12 copies/ cell per month from 0 to 6 months (P = 0.3 7) and 2 6.96 copies/cel I per month from 6 to 12 months (P < 0.0001)].Conclusions: This study is the first showing that mitochondrial DNA content can increase in peripheral blood lymphocytes during treatment interruption, but only after at least 6 months of interruption. Consequently, interruptions of shorter periods, whether by clinician or patient decision, are unlikely to allow restoration of mitochondrial DNA and so decrease HAART-related toxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|