Small noncoding RNA modulates japanese encephalitis virus replication and translation in trans
| Autor: | Yi-Hsin Fan, M. S. A. Muthukumar Nadar, Chia-Chen Weng, Ruey-Yi Chang, Yun-Tong Lin, Chiu-Chin Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Untranslated region
Gene Expression Regulation Viral viruses RNA-dependent RNA polymerase Biology Real-Time Polymerase Chain Reaction Virus Replication Models Biological Virus Cell Line lcsh:Infectious and parasitic diseases Cricetinae Virology medicine Animals lcsh:RC109-216 Subgenomic mRNA Genetics Encephalitis Virus Japanese Research RNA Japanese encephalitis medicine.disease Non-coding RNA Blotting Northern Infectious Diseases Viral replication Protein Biosynthesis RNA Small Untranslated RNA Viral |
| Zdroj: | Virology Journal, Vol 8, Iss 1, p 492 (2011) Virology Journal |
| Popis: | Background Sequence and structural elements in the 3'-untranslated region (UTR) of Japanese encephalitis virus (JEV) are known to regulate translation and replication. We previously reported an abundant accumulation of small subgenomic flaviviral RNA (sfRNA) which is collinear with the highly conserved regions of the 3'-UTR in JEV-infected cells. However, function of the sfRNA in JEV life cycle remains unknown. Results Northern blot and real-time RT-PCR analyses indicated that the sfRNA becomes apparent at the time point at which minus-strand RNA (antigenome) reaches a plateau suggesting a role for sfRNA in the regulation of antigenome synthesis. Transfection of minus-sense sfRNA into JEV-infected cells, in order to counter the effects of plus-sense sfRNA, resulted in higher levels of antigenome suggesting that the presence of the sfRNA inhibits antigenome synthesis. Trans-acting effect of sfRNA on JEV translation was studied using a reporter mRNA containing the luciferase gene fused to partial coding regions of JEV and flanked by the respective JEV UTRs. In vivo and in vitro translation revealed that sfRNA inhibited JEV translation. Conclusions Our results indicate that sfRNA modulates viral translation and replication in trans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|