Physiological and molecular osmotic stress responses in three durum wheat (Triticum turgidum ssp durum) genotypes
| Autor: | Giorgia Capasso, Giorgia Santini, Sergio Esposito, Sawsen Ayadi, Fatma Ezzahra Ben Azaiez, Youssef Trifa, Zoubeir Chamekh, Salma Jallouli, Simone Landi, Ines Zouari |
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| Přispěvatelé: | Jallouli, Salma, Ayadi, Sawsen, Landi, Simone, Capasso, Giorgia, Santini, Giorgia, Chamekh, Zoubeir, Zouari, Ine, Azaiez Fatma Ezzhara, Ben, Trifa, Youssef, Esposito, Sergio |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Osmotic stress Osmotic shock Growth performance Dehydrogenase 01 natural sciences lcsh:Agriculture 03 medical and health sciences Glutamine synthetase Glutamate synthase Durum wheat 030304 developmental biology 0303 health sciences biology Chemistry Glutamate dehydrogenase drought stress lcsh:S APX Glutamine Biochemistry biology.protein Drought stre Phosphoenolpyruvate carboxylase Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Agronomy, Vol 9, Iss 9, p 550 (2019) Agronomy Volume 9 Issue 9 |
| Popis: | This study aims to investigate the activities and expression of enzymes of primary metabolism and relate these data with the growth performance of three different durum wheat genotypes (Maali YT13 and ON66) under osmotic stress. Growth traits&mdash including plant height, dry weight (DW) and relative water content (RWC)&mdash were measured to classify genotypes depending on their tolerance to stress. Several enzymes were investigated: Ascorbate peroxidase (APX), Glutamine Synthetase (GS), Glutamine dehydrogenase (GDH), Glutamate synthase (GOGAT), Glucose 6-phosphate dehydrogenase (G6PDH), and Phosphoenolpyruvate Carboxylase (PEPC). The expression of the cytosolic and plastidic glutamine synthetase (TaGS1 and TaGS2), high affinity nitrate transporters (TaNRT2.3) and Glutamate dehydrogenase (TaGDH) were also detected by qRT-PCR. The results indicated different growth performances among genotypes, indicating Maali and YT13 as tolerant genotypes and ON66 as a drought-susceptible variety. Data showed a decrease in PEPC and increase in APX activities under osmotic stress a slight decrease in GS activity was observed, together with an increase in G6PDH in all genotypes GS and NRT2 expressions changed in a similar pattern in the different genotypes. Interestingly, Maali and YT13 showed higher transcript abundance for GDH under stress compared to ON66, suggesting the implication of GDH in protective phenomena upon osmotic stress. |
| Databáze: | OpenAIRE |
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