Sequence-Specific Crosslinking of Electrospun, Elastin-Like Protein Preserves Bioactivity and Native-Like Mechanics

Autor:	Krishna Prasad Chennazhi, Helen Fink, Jeffrey A. Sweet, Annika Enejder, Patrick L. Benitez, Shantikumar V. Nair, Sarah C. Heilshorn
Rok vydání:	2012
Předmět:	Stromal cell Materials science Rotation Molecular Sequence Data Biomedical Engineering Pharmaceutical Science Biocompatible Materials Sequence (biology) macromolecular substances 02 engineering and technology Protein Engineering Article Biomaterials Extracellular matrix 03 medical and health sciences Biomimetic Materials Elastic Modulus Materials Testing Cell Adhesion Electrochemistry Animals Humans Amino Acid Sequence Cell adhesion 030304 developmental biology 0303 health sciences biology technology industry and agriculture Protein engineering Adhesion Mechanics 021001 nanoscience & nanotechnology Elastin Extracellular Matrix 3. Good health Cross-Linking Reagents Nanofiber biology.protein 0210 nano-technology Oligopeptides
Zdroj:	Advanced Healthcare Materials. 2:114-118
ISSN:	2192-2640
DOI:	10.1002/adhm.201200115
Popis:	A nanoscale mimic of the extracellular matrix is electrospun from a highly tunable family of elastin-like proteins. A sequence-specific, two-step crosslinking procedure is developed to preserve the nanofiber morphology, elastin-like mechanics, and specific bioactivity. Rodent marrow stromal cells show sequence-specific adhesion on the matrices, which are imaged using label-free coherent anti-Stokes Raman scattering (CARS) microscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f36b8f14ff92b9acb1fcff5ef52432d https://doi.org/10.1002/adhm.201200115 Zobrazit plný text záznamu Plný text