Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin
| Autor: | Maria do Rosário Rodrigues Silva, Marize Campos Valadares Bozinis, Orionalda de Fátima Lisboa Fernandes, Alessandro Lopes da Silva, Cecília M. A. de Oliveira, Lucilia Kato, Wanessa Machado Andrade, Lúcia Kioko Hasimoto e Souza, Carolina Rodrigues Costa, Maysa Paula da Costa |
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| Jazyk: | angličtina |
| Předmět: |
Neutral red
Antifungal Agents Flavonols Cytotoxicity Microbial Sensitivity Tests Hymenaea courbaril Biology Cell Line chemistry.chemical_compound Mice Xylem Taxifolin Animals Food science Antifungal activity Fustin Flavonoids Arthrodermataceae Broth microdilution fungi food and beverages General Medicine biology.organism_classification Biochemistry chemistry Complementary and alternative medicine Cryptococcus neoformans Hymenaea Fisetin Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/1472-6882-14-245 |
| Popis: | Background The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. Methods The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. Results The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) |
| Databáze: | OpenAIRE |
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