Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2
| Autor: | Junsheng Feng, Andrew Baker, Jelica Gluhak-Heinrich, Lynn Wang, Li An Wu, Mary MacDougall, Shuo Chen, Yan Dong Mu, Kevin J. Donly, Stephen E. Harris |
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| Rok vydání: | 2010 |
| Předmět: |
Physiology
Cellular differentiation Clinical Biochemistry Mesenchymal cell differentiation Bone Morphogenetic Protein 2 Mice Transgenic Biology Article Cell Line Mice Calcification Physiologic stomatognathic system Animals Dental papilla Cell Shape Dental Papilla Odontoblasts Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Anatomy Cell biology stomatognathic diseases Phenotype Odontoblast Dentinogenesis Dentin mineralization Immortalised cell line Biomarkers |
| Zdroj: | Journal of Cellular Physiology. 225:132-139 |
| ISSN: | 0021-9541 |
| Popis: | Bone morphogenetic protein 2 (Bmp2) is essential for odontogensis and dentin mineralization. Generation of floxed Bmp2 dental mesenchymal cell lines is a valuable application for studying the effects of Bmp2 on dental mesenchymal cell differentiation and its signaling pathways during dentinogenesis. Limitation of the primary culture of dental mesenchymal cells has led to the development of cell lines that serve as good surrogate models for the study of dental mesenchymal cell differentiation into odontoblasts and mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines, which were isolated from 1st mouse mandibular molars at postnatal day 1 and immortalized with pSV40 and clonally selected. These transfected cell lines were characterized by RT-PCR, immunohistochemistry, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized cell lines, iBmp2-dp, displayed a higher proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary cells as determined by expression of tooth-specific markers as well as demonstrated the ability to differentiate and form mineralized nodules. In addition, iBmp2-dp cells were inducible and responded to BMP2 stimulation. Thus, we for the first time described the establishment of an immortalized mouse floxed Bmp2 dental papilla mesenchyma cell line that might be used for studying the mechanisms of dental cell differentiation and dentin mineralization mediated by Bmp2 and other growth factor signaling pathways. |
| Databáze: | OpenAIRE |
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