Berberine enhances chemosensitivity to irinotecan in colon cancer via inhibition of NF-κB
| Autor: | Lingzhi Wang, Naiju Zhang, Meiling Yu, Cuiling Zhang, Benquan Qi, Binbin Yu, Nan Tang, Xu-hui Tong, Shuying Dong, Hongdang Qu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Small interfering RNA Berberine Survivin bcl-X Protein Down-Regulation Antineoplastic Agents Apoptosis Irinotecan Biochemistry Inhibitor of Apoptosis Proteins chemistry.chemical_compound Genetics Humans Medicine heterocyclic compounds Doxorubicin RNA Small Interfering neoplasms Molecular Biology Oncogene business.industry NF-kappa B Transcription Factor RelA NF-κB HCT116 Cells Molecular biology digestive system diseases Oncology chemistry Colonic Neoplasms Cancer research Molecular Medicine Camptothecin RNA Interference Fluorouracil Apoptosis Regulatory Proteins business medicine.drug |
| Zdroj: | Molecular Medicine Reports. 9:249-254 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2013.1762 |
| Popis: | Previous studies have shown that irinotecan (CPT‑11) impairs chemotherapy‑induced apoptosis by activating nuclear factor‑κB (NF‑κB) and a number of strategies have been employed to augment chemosensitivity through the suppression of NF‑κB activation. Berberine, a botanical alkaloid, was reported to enhance chemosensitivity to 5‑fluorouracil and doxorubicin by suppressing NF‑κB activation. In the present study, the effect of berberine on CPT‑11‑induced apoptosis was investigated through the inhibition of NF‑κB. Inhibition of NF‑κB activation by p65 small interfering RNA was shown to potentiate apoptosis induced by CPT‑11. Berberine suppressed CPT‑11‑induced NF‑κB activation in a dose‑dependent manner and enhanced chemosensitivity to CPT‑11 by downregulating NF‑κB activation of antiapoptotic genes, c‑IAP1, c‑IAP2, survivin and Bcl‑xL. The current observations indicate that berberine inhibits NF‑κB activation and may be used to enhance CPT‑11‑induced apoptosis in colon cancer. |
| Databáze: | OpenAIRE |
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