Stabilization of cell-free RNA in blood samples using a new collection device
| Autor: | Sheila E. Norton, Joel M. Lechner, Jianbing Qin, Kristin K. Luna, M. Rohan Fernando |
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| Rok vydání: | 2012 |
| Předmět: |
Male
RNA Stability Cell Survival Clinical Biochemistry Biology Real-Time Polymerase Chain Reaction 18S ribosomal RNA Ribonucleases RNA Ribosomal 18S Humans RNA Messenger Enzyme Inhibitors Edetic Acid Messenger RNA Blood Specimen Collection Blood Cells RNA Genes fos Glyceraldehyde-3-Phosphate Dehydrogenases General Medicine Ribosomal RNA Molecular biology Reverse transcriptase Actins Real-time polymerase chain reaction Blood Preservation Female Ex vivo |
| Zdroj: | Clinical biochemistry. 45(16-17) |
| ISSN: | 1873-2933 |
| Popis: | Objective To investigate whether a new blood collection device stabilizes cell-free RNA (cfRNA) in blood post-phlebotomy when compared to collection using K3EDTA tubes. Design and methods Blood samples were drawn from healthy donors into K3EDTA tubes and Cell-Free RNA BCTs (BCTs) and stored at room temperature (20–25 °C). At specified time points (days 0–3), plasma was separated and cfRNA was extracted. Reverse transcription real-time PCR was used to quantify mRNA for c-fos, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and for 18S rRNA. Results Blood drawn into K3EDTA tubes showed a steady increase in RNA concentration over 3 days of ex vivo incubation. Blood drawn into BCTs showed no statistically significant change in RNA copy number except for GAPDH on day 3. Conclusions The novel chemical cocktail contained in the new device allows for the stabilization of cfRNA in blood samples at room temperature, which potentially enhances the clinical utility of cfRNA. |
| Databáze: | OpenAIRE |
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