Serotonin receptors are selectively expressed in the avian germ cells and early embryos
| Autor: | U. Stepinska, Takashi Kuwana, B. Olszanska |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Embryo Nonmammalian Embryogenesis Gene Expression Regulation Developmental Embryo Coturnix Gastrula Cell Biology Biology Oocyte Spermatozoa Molecular biology Cell biology Gastrulation medicine.anatomical_structure Hormone receptor Receptors Serotonin Oocytes medicine Animals Female Germ line development Receptor 5-HT receptor Developmental Biology |
| Zdroj: | Zygote. 23:394-405 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199413000683 |
| Popis: | SummaryThe expression of nine serotonin (5-HT) receptor transcripts was studied using reverse transcription polymerase chain reaction (RT-PCR) in germ cells, cleavage and gastrulation stages of Japanese quail, and qPCR for 5-HT3and 5-HT4receptors in oocytes and embryos. We show the presence/absence of nine serotonin transcripts known in birds for receptors 5-HT1A, 5-HT1F, 5-HT2B, 5-HT2C, 5-HT3, 5-HT4, 5-HT5A, 5-HT6and 5-HT7Ain avian germ cells and early embryos. The absence of 5-HT3and 5-HT5Ain primordial germ cells and of 5-HT3and 5-HT7Ain sperm is characteristic. All transcripts appeared in oocytes at all stages (except for 5-HT3and 5-HT5Atranscripts) and all were present in cleaving embryos and at gastrulation, except for 5-HT3, which was permanently observed as late as in stage 4. Interestingly, 5-HT3and 5-HT5Areceptors accumulated in 3-mm and F1 oocytes but were degraded at ovulation and started to be re-transcribed in cleavage stage II embryos and beyond. The selective appearance of 5-HT receptors in germ cells and early embryos supports the hypothesis that serotonin may act as a signalling molecule at early stages of germ line and embryo differentiation via individual receptors present during different stages, when specialized communication systems are not yet developed. |
| Databáze: | OpenAIRE |
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