Aspergillus nidulans nuclear proteins bind to a CCAAT element and the adjacent upstream sequence in the promoter region of the starch-inducible Taka-amylase A gene
| Autor: | Norihiro Tsukagoshi, Osamu Nagata, Tomoko Takashima, Makoto Tanaka |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Fungal Molecular Sequence Data CAAT box Cell Fractionation Aspergillus nidulans Fungal Proteins Structure-Activity Relationship Gene Expression Regulation Fungal Consensus Sequence Gene expression Genetics Consensus sequence Promoter Regions Genetic Molecular Biology Gene Cell Nucleus Base Sequence biology Nucleic acid sequence Nuclear Proteins RNA Fungal Starch Promoter biology.organism_classification Molecular biology DNA-Binding Proteins Regulatory sequence Enzyme Induction CCAAT-Enhancer-Binding Proteins alpha-Amylases |
| Zdroj: | Molecular and General Genetics MGG. :251-260 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00282807 |
| Popis: | Aspergillus nidulans was used as an intermediate host to investigate the regulation of the Taka-amylase A (TAA) gene from Aspergillus oryzae. The induction of Taa by starch was confirmed to be regulated at the transcriptional level by analyzing the transcripts specific for Taa synthesized in vitro in nuclei from starch- and glucose-grown cells. A 55 bp DNA fragment containing a consensus CCAAT sequence from the promoter region of the Taa gene was shown to confer starch inducibility on the gene. A nuclear extract from starch-grown cells was assayed for proteins which bind to the promoter region of the Taa gene. A protein designated AnCP1 bound to the CCAAT sequence. A nuclear extract from glucose-grown cells contained two DNA-binding proteins designated AnCP2 and AnNP1. AnCP2 bound to the same CCAAT sequence as AnCP1, while AnNP1 bound to the 25 bp region just upstream of the AnCP2 binding site. Occupancy of the two binding sites appeared to be mutually exclusive, which is suggestive of a negative regulatory mechanism for gene expression. |
| Databáze: | OpenAIRE |
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