Bone morphogenetic protein (BMP)1-3 enhances bone repair
| Autor: | Marija Lipar, Vera Kufner, Marko Stejskal, Dragan Durdevic, Stefan Prgomet, Ivo Dumić-Čule, Drazen Maticic, Drazen Vnuk, Lovorka Grgurevic, Tomislav Smoljanović, Jelena Brkljacic, Igor Erjavec, Mladen Mercep, Slobodan Vukicevic, Boris Macek, Mislav Jelić |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Bone morphogenetic protein 8A Biophysics Bone healing Biochemistry Bone morphogenetic protein 2 Bone and Bones Collagen Type I Bone Morphogenetic Protein 1 Cell Line Rats Sprague-Dawley Fractures Bone Mice Osteogenesis Bone cell Animals Humans BMP1-3 Bone fracture ECM BMP7 Differentiation Molecular Biology Wound Healing Osteoblasts Chemistry Bone morphogenetic protein 10 Cell Differentiation Cell Biology Recombinant Proteins Rats Cell biology Bone morphogenetic protein 7 Bone morphogenetic protein 6 Bone morphogenetic protein 5 Immunology Rabbits |
| DOI: | 10.1016/j.bbrc.2011.03.109 |
| Popis: | Members of the astacin family of metalloproteinases such as human bone morphogenetic protein 1 (BMP-1) regulate morphogenesis by processing precursors to mature functional extracellular matrix (ECM) proteins and several growth factors including TGFβ, BMP2, BMP4 and GFD8. We have recently discovered that BMP1-3 isoform of the Bmp-1 gene circulates in the human plasma and is significantly increased in patients with acute bone fracture. We hypothesized that circulating BMP1-3 might have an important role in bone repair and serve as a novel bone biomarker. When administered systemically to rats with a long bone fracture and locally to rabbits with a critical size defect of the ulna, recombinant human BMP1-3 enhanced bone healing. In contrast, neutralization of the endogenous BMP1-3 by a specific polyclonal antibody delayed the bone union. Invitro BMP1-3 increased the expression of collagen type I and osteocalcin in MC3T3-E(1) osteoblast like cells, and enhanced the formation of mineralized bone nodules from bone marrow mesenchymal stem cells. We suggest that BMP1-3 is a novel systemic regulator of bone repair. |
| Databáze: | OpenAIRE |
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