Molecular characterization and expression analysis of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE gene family in Paeonia suffruticosa
| Autor: | Shunli Wang, Xiuxia Ren, Xiuxin Zhang, Jingqi Xue, Xiaodan Cheng, Xiaogai Hou, Yuqian Xue |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Plant Science Flowers Paeonia 01 natural sciences Evolution Molecular 03 medical and health sciences Gene Expression Regulation Plant Gene family Squamosa promoter binding protein Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Transcription factor Gene Conserved Sequence Phylogeny Plant Proteins Genetics biology Phylogenetic tree Paeonia suffruticosa Promoter General Medicine biology.organism_classification MicroRNAs 030104 developmental biology Multigene Family Seeds Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant cell reports. 39(11) |
| ISSN: | 1432-203X |
| Popis: | A total of 16 PsSPL genes were identified in tree peony. PsSPLs potentially regulated flowering time, lateral bud and seed development, and the juvenile-to-adult phase transition. SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors are important for plant growth and development. Here, we report the identification of 16 full-length PsSPLs in tree peony (Peaonia suffruticosa Andr.) and 9 PsSPLs that have miR156 target sites. Phylogenetic analysis of the relationship of SPLs in P. suffruticosa and Arabidopsis suggested that they can be classified into six groups, and PsSPLs were highly correlated with Arabidopsis SPLs counterparts in the same group. Cis-element of promoter region analysis suggested that PsSPL genes play roles in physiological processes and developmental events. Expression analysis indicated that most PsSPL genes exhibited high expression levels in the tissues and organs examined here. The increasing expression levels of PsSPL1, PsSPL2, PsSPL8, PsSPL9, PsSPL12, and PsSPL16, and decreasing expression levels of PsSPL1A and PsSPL1B in buds over time suggested that they were probably regulated by the juvenile-to-adult phase transition. In addition, the expression profiles of PsSPL genes in different developmental buds and seeds suggested that PsSPL2, PsSPL3, PsSPL9, PsSPL10, PsSPL13, and PsSPL13A were important genes for regulating the flowering time of the tree peony; PsSPL2 and PsSPL8 might play a role in suppressing lateral bud development, and PsSPL2, PsSPL13, and PsSPL14 positively controlled grain size and number, and pod branching. These results provide a foundation for future functional analysis of PsSPL genes in tree peony growth and development. |
| Databáze: | OpenAIRE |
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