The alpha 1-subunit of skeletal muscle L-type Ca channels is the key target for regulation by A-kinase and protein phosphatase-1C
| Autor: | Xiao-Ian Zhao, Chan Fong Chang, M. Marlene Hosey, L. M. Gutierrez |
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| Rok vydání: | 1994 |
| Předmět: |
inorganic chemicals
Gene isoform Phosphopeptides Macromolecular Substances Phosphatase Biophysics Biochemistry Peptide Mapping Substrate Specificity Dephosphorylation medicine Phosphoprotein Phosphatases Animals Protein phosphorylation Electrophoresis Gel Two-Dimensional Phosphorylation Protein kinase A Molecular Biology Ion channel Chemistry Muscles Myocardium Cell Membrane Skeletal muscle Cell Biology Phosphoproteins Cyclic AMP-Dependent Protein Kinases Kinetics medicine.anatomical_structure Cattle Calcium Channels Rabbits |
| Zdroj: | Biochemical and biophysical research communications. 198(1) |
| ISSN: | 0006-291X |
| Popis: | Despite the fact that the phosphorylation-mediated regulation of L-type Ca channels is viewed as a model of ion channel regulation, much remains to be learned about the protein phosphorylation and dephosphorylation reactions that underlie the regulation of the channels. The channel isoform most well studied biochemically is that expressed in skeletal muscle. The alpha 1- and beta-subunits of this channel isoform are substrates for protein kinase A, but it is unknown if phosphorylation or dephosphorylation of both subunits contributes to altered channel properties. Here, we report experiments in which the alpha 1- and beta-subunits were differentially phosphorylated by protein kinase A and dephosphorylated by protein phosphatase 1c under conditions that led to channel regulation. The results suggest that the alpha 1-subunit plays a key role in the phosphorylation and dephosphorylation-dependent regulation of the L-type Ca channels from skeletal muscle. |
| Databáze: | OpenAIRE |
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