Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4
| Autor: | Kenichi Iwata, Toshio Omori, Hironori Yamakawa, Azlin Azlan |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Inorganic chemistry
chemistry.chemical_element Bioengineering Lysobacter DNA Ribosomal Polymerase Chain Reaction Applied Microbiology and Biotechnology Ammonia chemistry.chemical_compound Nitrogen Fixation RNA Ribosomal 16S Ammonium Food science Incubation DNA Primers Base Sequence biology Strain (chemistry) Temperature Hydrogen-Ion Concentration biology.organism_classification Nitrogen Carbon Culture Media Glucose chemistry Nitrogen fixation Bacteria Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 110:415-418 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2010.05.006 |
| Popis: | This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (∼1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron. |
| Databáze: | OpenAIRE |
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