Suppression of viral specific primary T-cell response following intense physical exercise in young but not old mice
| Autor: | Michael L. McRae, Rafi Ahmed, Zoher F. Kapasi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD4-Positive T-Lymphocytes
Aging Physiology Ratón Physical exercise CD8-Positive T-Lymphocytes Lymphocytic Choriomeningitis Lymphocyte Activation Lymphocytic choriomeningitis Mice Immune system Physical Conditioning Animal Physiology (medical) MHC class I medicine Animals Lymphocytic choriomeningitis virus Cytotoxic T cell biology T lymphocyte medicine.disease Mice Inbred C57BL Immunology Physical Endurance biology.protein Female CD8 |
| Zdroj: | Journal of Applied Physiology. 98:663-671 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.00510.2004 |
| Popis: | Intense exercise to exhaustion leads to increased susceptibility and severity of infections. T cells play an essential role in control of viral infections. Whereas immune suppression is considered as a likely mechanism for exhaustive exercise-induced susceptibility to infection, we know little about viral-specific T-cell response following exhaustive exercise in young or old mice. In this study, one group of female young (10–12 wk) and old (22–24 mo) C57BL/6 mice was exposed to a single bout of intense exercise to exhaustion and immediately infected with lymphocytic choriomeningitis virus (LCMV). Eight days later, at the peak of expansion phase of T-cell response, we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells and a sensitive functional assay measuring interferon-γ production at the single-cell level to quantitate the CD8 and CD4 T-cell response. To evaluate the impact of intense exercise during both the initiation and evolution of the expansion phase of the T-cell response, a second group of young and old mice continued their daily bouts of intense exercise to exhaustion over the next 8 days. Our data show that, in young mice, LCMV infection following exhaustive exercise leads to suppression of LCMV-specific CD8 and CD4 T-cell responses, and this suppression effect occurs at the initiation of the expansion phase of viral-specific T cells. However, in old mice, unlike young mice, exhaustive exercise does not cause suppression of LCMV-specific T-cell responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|