Identification of miRNAs from stem cell derived microparticles in umbilical cord blood
| Autor: | Dimitris Bougiouklis, Antonia Sioga, Achilles Anagnostopoulos, Marina Gerousi, Anagnostis Argiriou, Damianos Sotiropoulos, Angeliki Xagorari |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
congenital hereditary and neonatal diseases and abnormalities Cancer Research CD34 Antigens CD34 Real-Time Polymerase Chain Reaction Umbilical cord Flow cytometry Andrology 03 medical and health sciences 0302 clinical medicine Cell-Derived Microparticles microRNA Genetics medicine Humans Annexin A5 skin and connective tissue diseases Molecular Biology medicine.diagnostic_test Chemistry Infant Newborn nutritional and metabolic diseases Cell Biology Hematology Fetal Blood Hematopoietic Stem Cells Transplantation Red blood cell MicroRNAs Microscopy Electron 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cord blood Cord Blood Stem Cell Transplantation Stem cell |
| Zdroj: | Experimental hematology. 80 |
| ISSN: | 1873-2399 |
| Popis: | Umbilical cord blood CD34+ (UCB-CD34+) stem cells are clinically used in hematopoietic cell transplantation. However, there are limitations in the use of umbilical cord blood transplants because of the small number of cells and delayed engraftment. To gain a better understanding of functional components of UCB, we have detected and characterized CD34+ microparticles (CD34+MPs) from cord blood units. We collected cord blood units and assessed the numbers of CD34+MPs before and after red blood cell and plasma depletion by SEPAX processing using flow cytometry analysis. In parallel we identified MPs by electron microscopy. CD34+MPs and cells were isolated by MACs sorting. MicroRNAs (miR-106, miR-221, miR-517, miR-519, and miR-221) exhibited a characteristic microRNA profile that was further validated in isolated CD34+MPs. We found that in cord blood, there are CD34+MPs that carry microRNAs. |
| Databáze: | OpenAIRE |
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