DNAzyme Sensor Uses Chemiluminescence Resonance Energy Transfer for Rapid, Portable, and Ratiometric Detection of Metal Ions
| Autor: | Siu Yee New, Ryan J. Lake, Jiao Zheng, Yi Lu, Jing Luen Wai, Zhike He |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Ions Luminescence Chemistry Metal ions in aqueous solution Deoxyribozyme Biosensing Techniques DNA Catalytic Hydrogen Peroxide Photochemistry Fluorescence Article Analytical Chemistry Luminol law.invention G-Quadruplexes chemistry.chemical_compound Energy Transfer law Fluorometer Hemin Chemiluminescence |
| Zdroj: | Anal Chem |
| ISSN: | 1520-6882 |
| Popis: | DNAzymes have emerged as an important class of sensors for a wide variety of metal ions, with florescence DNAzyme sensors as the most widely used in different sensing and imaging applications because of their fast response time, high signal intensity, and high sensitivity. However, the requirements of an external excitation light source and its associated power increase the cost and size of the fluorometer, making it difficult to be used for portable detections. To overcome these limitations, we report herein a DNAzyme sensor that relies on chemiluminescence resonance energy transfer (CRET) without the need for external light. The sensor is constructed by combining the functional motifs from both Pb(2+)-dependent 8–17 DNAzyme conjugated to fluorescein (FAM) and hemin/G-quadruplex that mimics horseradish peroxidase to catalyze the oxidation of luminol by H(2)O(2) to yield chemiluminescence. In the absence of Pb(2+), the hybridization between the enzyme and substrate strands bring the FAM and hemin/G-quadruplex in close proximity, resulting in CRET. The presence of Pb(2+) ions can drive the cleavage on the substrate strand, resulting in a sharp decrease in the melting temperature of hybridization and thus separation of the FAM from hemin/G-quadruplex. The liberated CRET pair causes a ratiometric increase in the donor’s fluorescent signal and a decrease in the acceptor signal. Using this method, Pb(2+) ions have been measured rapidly ( |
| Databáze: | OpenAIRE |
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