Effect of Hypothalamic Proline-Rich Peptide (PRP-1) on Neuronal and Bone Marrow Cell Apoptosis
| Autor: | Armen A. Galoyan, Susanne Klumpp, Josef Krieglstein, Wolfram Kremers, Kristina E. Danielian, Tigran K. Davtyan, Karine A. Galoian, Kristina B. Bezirganyan |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Apoptosis Bone Marrow Cells Granulocyte Biology Hippocampus Biochemistry Neuroprotection Cellular and Molecular Neuroscience Internal medicine medicine Animals Staurosporine Neurons Monocyte General Medicine Rats Haematopoiesis Endocrinology medicine.anatomical_structure Doxorubicin Proline-Rich Protein Domains Myelopoiesis Bone marrow Peptides medicine.drug |
| Zdroj: | Neurochemical Research. 32:1898-1905 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-007-9379-9 |
| Popis: | The AGAPEPAEPAQPGVY proline-rich peptide (PRP-1) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. It has been shown that PRP-1 has many potentially beneficial biological effects including immunoregulatory, hematopoietic, antimicrobial and anti-neurodegenerative properties. Here we investigated the influence of PRP-1 on staurosporine-induced apoptosis of postnatal hippocampal cells and on doxorubicin-induced bone marrow granulocyte- and monocyte apoptosis. The intention was to further characterize the effect of PRP-1 on the survival rate of neurons and in context with myelopoiesis. We demonstrate that PRP-1 significantly reduced apoptosis of postnatal hippocampal cells induced by staurosporine. The protective effect of PRP-1 against apoptotic cell death was shown to be both time- and dose-dependent. Neuroprotection was more pronounced after prolonged pretreatment of the cells with PRP-1 before the induction of apoptosis with staurosporine. The related peptide [arg(8)]vasopressin did not reveal neuroprotection. PRP-1 also significantly reduced apoptosis of bone marrow monocytes and granulocytes induced by doxorubicin. This protective effect lasted for 2-4 h and was not detectable anymore after 24 h when PRP-1 and doxorubicin were added simultaneously. Previously obtained data and results of the current studies suggested that the hypothalamic PRP-1 possibly represents an endogenous peptide whose primary functions are to regulate myelopoiesis and neuron survival as we provide evidence that PRP can differentially reduce both staurosporine- and doxorubicin-induced hippocampal and bone marrow cell apoptosis. |
| Databáze: | OpenAIRE |
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