Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania
| Autor: | Samuel Maselle, Peter C Gøtzsche, Ernest Yangi, Jesper Svendsen, Peter Skinhøj, Viggo Faber, Anders F. Jørgensen, Annemette Thomsen, Ib C. Bygbjerg, Sylvester Matunda, John F. Shao |
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| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Blotting Western Human immunodeficiency virus (HIV) Enzyme-Linked Immunosorbent Assay HIV Infections HIV Antibodies medicine.disease_cause Tanzania Health services Predictive Value of Tests medicine False positive paradox Humans False Positive Reactions False Negative Reactions Observer Variation Hematologic tests General Immunology and Microbiology biology Obstetrics business.industry nutritional and metabolic diseases General Medicine biology.organism_classification Infectious Diseases Interobserver Variation Immunology business Indeterminate |
| Zdroj: | Scandinavian journal of infectious diseases. 22(3) |
| ISSN: | 0036-5548 |
| Popis: | 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded. |
| Databáze: | OpenAIRE |
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