Hidden Carbapenem Resistance in OXA-48 and Extended-Spectrum β-Lactamase-PositiveEscherichia coli
| Autor: | Branka Bedenić, Domagoj Drenjančević, Maja Tomić Paradžik, Aleksandra Presečki-Stanko, Jasminka Talapko, Jasminka Kopić, Gernot Zarfel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Carbapenem medicine.drug_class Immunology Cephalosporin Biology medicine.disease_cause Microbiology 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Antibiotic resistance Plasmid polycyclic compounds medicine Escherichia coli 030304 developmental biology Pharmacology 0303 health sciences 030306 microbiology biochemical phenomena metabolism and nutrition bacterial infections and mycoses chemistry bacteria Multilocus sequence typing β-lactamases ESBL carbapenemases OXA-48 Ertapenem medicine.drug |
| Zdroj: | Microbial Drug Resistance. 25:696-702 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2018.0309 |
| Popis: | The purpose of this study was to report the identification OXA-48 carbapenemase in seven extended-spectrum β-lactamase (ESBL)-positive Escherichia coli clinical isolates, fully susceptible to all carbapenems by disk diffusion and E-test methods, but with borderline minimal inhibitory concentration (MIC) values of ertapenem. This report points to the necessity for determination of carbapenem MICs in ESBL-positive E. coli isolates and additional phenotypic testing for carbapenemases in all isolates with borderline ertapenem MIC defined by EUCAST. The isolates showed a high level of resistance to expanded- spectrum cephalosporins because of the production of an additional ESBL belonging to CTX-M family. All isolates and their respective tranconjugants were found to possess L plasmid. Pulsed-field gel electrophoresis analysis revealed two clusters containing highly related isolates. The global spread of multidrug-resistant E. coli should be monitored closely because of the ability of isolates to rapidly obtain additional antibiotic resistance traits such as plasmid-mediated OXA-48 genes. |
| Databáze: | OpenAIRE |
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