Core-shell structured magnetic metal-organic framework composites for highly selective enrichment of endogenous N-linked glycopeptides and phosphopeptides
| Autor: | Yonglei Wu, Qianjing Liu, Chunhui Deng, Yiqin Xie |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Phosphopeptides
Proteomics Ligand Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry Glycopeptides 02 engineering and technology 021001 nanoscience & nanotechnology Mass spectrometry Highly selective 01 natural sciences Ferrosoferric Oxide Glycopeptide 0104 chemical sciences Analytical Chemistry Metal visual_art visual_art.visual_art_medium Metal-organic framework Composite material 0210 nano-technology Selectivity Metal-Organic Frameworks |
| Zdroj: | Talanta. 190:298-312 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2018.08.010 |
| Popis: | In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe3O4 @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand. The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC. Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect