Dissecting the Genetic Architecture of Shoot Growth in Carrot (Daucus carota L.) Using a Diallel Mating Design
| Autor: | Brian S. Yandell, Paul L. Maurizio, William Valdar, Sarah D. Turner, Philipp W. Simon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
0106 biological sciences Canopy MPP multiple imputation Heterosis degree of dominance Growing season QH426-470 01 natural sciences diallel Bayesian mixed model Diallel cross 03 medical and health sciences heterosis Genetics Plant breeding Molecular Biology Genetics (clinical) Hybrid 2. Zero hunger biology food and beverages 04 agricultural and veterinary sciences Mating design Heritability 15. Life on land biology.organism_classification genetic architecture 030104 developmental biology Agronomy Shoot multiparental populations 040103 agronomy & agriculture 0401 agriculture forestry and fisheries 010606 plant biology & botany Daucus carota |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 8, Iss 2, Pp 411-426 (2018) |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.117.300235 |
| Popis: | Crop establishment in carrot (Daucus carotaL.) is limited by slow seedling growth and delayed canopy closure, resulting in high management costs for weed control. Varieties with improved growth habit (i.e. larger canopy and increased shoot biomass) may help mitigate weed control, but the underlying genetics of these traits in carrot is unknown. This project used a diallel mating design coupled with recent Bayesian analytical methods to determine the genetic basis of carrot shoot growth. Six diverse carrot inbred lines with variable shoot size were crossed in WI in 2014. F1 hybrids, reciprocal crosses, and parental selfs were grown in a randomized complete block design (RCBD) with two blocks in CA (2015, 2016) and in WI (2015). Measurements included canopy height, canopy width, shoot biomass, and root biomass. General and specific combining abilities were estimated using Griffing’s Model I. In parallel, additive, inbreeding, epistatic, and maternal effects were estimated from a Bayesian linear mixed model, which is more robust to dealing with missing data, outliers, and theoretical constraints than traditional biometric methods. Both additive and non-additive effects significantly influenced shoot traits, with non-additive effects playing a larger role early in the growing season, when weed control is most critical. Results suggest that early season canopy growth and root size express hybrid vigor and can be improved through reciprocal recurrent selection.Article SummaryBreeding for improved competitive ability is a priority in carrot, which suffers yield losses under weed pressure. However, improvement and in-depth genetic studies for these traits relies on knowledge of the underlying genetic architecture. This study estimated heritable and non-heritable components of carrot shoot growth from a diallel mating design using a Bayesian mixed model. Results directly contribute to improvement efforts by providing estimates of combining ability, identifying a useful tester line, and characterizing the genetic and non-genetic influences on traits for improved competitive ability in carrot. |
| Databáze: | OpenAIRE |
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