Development and characterization of microsatellite loci in the entocommensal Malacobdella arrokeana (Nemertea: Bdellonemertea), from Patagonia (Argentina) and cross-amplification in 34 nemertean species
| Autor: | Ricardo García-Jiménez, José E. F. Alfaya, Gregorio Bigatti, Violeta López-Márquez, Fernando Ángel Fernández-Álvarez, Annie Machordom |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Nemertea
POPULATION STRUCTURE biology Phylum Ecology Otras Ciencias Biológicas Endangered species Biodiversity Zoology Locus (genetics) biology.organism_classification Ciencias Biológicas PATAGONIA 454 PYROSEQUENCING Genetics Pyrosequencing Microsatellite Allele Ecology Evolution Behavior and Systematics CIENCIAS NATURALES Y EXACTAS NEMERTEAN |
| DOI: | 10.1007/s12686-013-0029-z |
| Popis: | Microsatellite loci (26 in total) were isolated for the first time for the entocommensal nemertean Malacobdella arrokeana, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying these markers in 8 multiplex and 5 individual reactions. One to 14 alleles were detected per locus across 25 samples analyzed from San Matías Gulf, Patagonia (Argentina). For the 25 polymorphic loci, observed and expected heterozygosities ranged from 0.125 to 0.920 and 0.119 to 0.890, respectively; five loci deviated from Hardy–Weinberg equilibrium. Limited distribution and host specificity of M.arrokeana, which only inhabits Panopea abbreviata, have endangered this nemertean given the unregulated commercial exploitation of its host. These useful markers provide data for future conservation strategies. Cross-amplifications were also tested in 34 nemerteans species, representing the major lineages in this phylum. In total, 18 of the 26 loci (from one to 11 per species) gave clear allelic profiles. Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: López Márquez, Violeta. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España Fil: García Jimenez, Ricardo. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España Fil: Fernández Álvarez, Fernando Ángel. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: Machordom Barbe, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España |
| Databáze: | OpenAIRE |
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