Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9
| Autor: | Lina Zhang, Shuang-ping Zhao, Yuhang Ai, Dongdong Wu, Hongyi Tan, Songyun Deng, Xiaoli Su, Pinhua Pan, Lemeng Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Interleukin-1beta mitochondrial DNA Pharmacology Systemic inflammation medicine.disease_cause p38 Mitogen-Activated Protein Kinases lcsh:Chemistry Pathogenesis Mice HMGB1 Protein lcsh:QH301-705.5 Spectroscopy systemic inflammation biology medicine.diagnostic_test lipopolysaccharide General Medicine respiratory system Computer Science Applications medicine.symptom Injections Intraperitoneal Lung injury HMGB1 DNA Mitochondrial Article Catalysis Inorganic Chemistry Sepsis 03 medical and health sciences medicine Animals Physical and Theoretical Chemistry Molecular Biology Interleukin-6 Organic Chemistry medicine.disease respiratory tract diseases Mice Inbred C57BL Toll-Like Receptor 4 030104 developmental biology Bronchoalveolar lavage acute lung injury lcsh:Biology (General) lcsh:QD1-999 Toll-Like Receptor 9 Immunology biology.protein TLR4 Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences, Vol 17, Iss 9, p 1425 (2016) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 9; Pages: 1425 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms17091425 |
| Popis: | The pathogenesis of sepsis is complex. Mitochondrial dysfunction, which is responsible for energy metabolism, intrinsic apoptotic pathway, oxidative stress, and systemic inflammatory responses, is closely related with severe sepsis induced death. Mitochondria DNA (mtDNA) contain un-methylated cytosine phosphate guanine (CpG) motifs, which exhibit immune stimulatory capacities. The aim of this study was to investigate the role and mechanism of mtDNA release on lipopolysaccharide (LPS) induced acute lung injury (ALI) and systemic inflammation. Following LPS injection, plasma mtDNA copies peak at 8 h. Compared with wild-type (WT) mice, mtDNA in toll like receptor 4 knockout (TLR4 KO) mice were significantly decreased. MtDNA intra-peritoneal administration causes apparent ALI as demonstrated by increased lung injury score, bronchoalveolar lavage fluid (BALF) total protein and wet/dry (W/D) ratio; mtDNA injection also directly provokes systemic inflammation, as demonstrated by increased IL-1β, IL-6, high-mobility group protein B1 (HMGB1) level; while nuclear DNA (nDNA) could not induce apparent ALI and systemic inflammation. However, compared with WT mice, TLR4 KO could not protect from mtDNA induced ALI and systemic inflammation. Specific TLR9 inhibitor, ODN 2088 pretreatment can significantly attenuate mtDNA induced ALI and systemic inflammation, as demonstrated by improved lung injury score, decreased lung wet/dry ratio, BALF total protein concentration, and decreased systemic level of IL-1β, IL-6 and HMGB1. MtDNA administration activates the expression of p-P38 mitogen-activated protein kinases (MAPK) in lung tissue and specific TLR9 inhibitor pretreatment can attenuate this activation. Thus, LPS-induced mtDNA release occurs in a TLR4-dependent manner, and mtDNA causes acute lung injury and systemic inflammation in a TLR9-dependent and TLR4-independent manner. |
| Databáze: | OpenAIRE |
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