Dynamics of CRISPR-Cas9 genome interrogation in living cells
| Autor: | Spencer C. Knight, Lea B. Witkowsky, Maxime Dahan, Mohamed El Beheiry, Lana Bosanac, Elisa T. Zhang, Zhe Liu, Robert Tjian, Jennifer A. Doudna, Liangqi Xie, Benjamin Guglielmi, Jean-Baptiste Masson, Wulan Deng |
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| Rok vydání: | 2015 |
| Předmět: |
Genetics
Multidisciplinary Genome Cas9 Heterochromatin Chromatin binding RNA Computational biology 3T3 Cells Biology Endonucleases Chromatin Mice Genome editing Bacterial Proteins CRISPR-Associated Protein 9 CRISPR Animals Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems DNA Cleavage Single-Cell Analysis Genetic Engineering |
| Zdroj: | Science (New York, N.Y.). 350(6262) |
| ISSN: | 1095-9203 |
| Popis: | Genome editing with a Cas9 scalpel The Cas9 nuclease forms the heart of the CRISPR-Cas genome editing system. Cas9 binds small guide RNAs that direct it to its target sites, where the nuclease either cleaves or binds to genomic DNA. Knight et al. used single-molecule imaging to track Cas9 in living cells. Cas9 searches the genome for its target sites using rapid threedimensional diffusion. It spends very little time binding to off-target sites, which explains the high accuracy of the CRISPRCas9 editing machine. Science , this issue p. 823 |
| Databáze: | OpenAIRE |
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