| Autor: |
Y. Loosli, Roman Muff, Jess G. Snedeker, Tomaso Zambelli, Guido Bartalena |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Biophysical Journal. 100:599a |
| ISSN: |
0006-3495 |
| Popis: |
A correlative link between decreased cancer cell stiffness and increased aggressiveness (metatstatic potential) has been established in a wide range of cancers, such as leukemia, breast cancer and chondrosarcoma. Nearly all studies establishing this link in adherent cells have utilized cell compression (indentation) or shearing (e.g. magnetic cyto-twisting). In this study we applied a novel tensile assay of cell stiffness to investigate the mechanical behavior of two osteosarcoma models (SAOS-2/LM5 and HUO9/M132), with each system comprising a parental cell line and its highly metastatic derivative form. The goal was to find a common trend linking cell stiffness and metastatic potential, further hypothesizing that a tensile assay could provide additional information compared to traditional compressive measures. Cells were therefore tested in two different mechanical configurations: in compression using atomic force micro-indentation; and in tension using a custom functional imaging platform that applies a controlled bi-axial deformation to the cell substrate while imaging and tracking substrate deformation at high spatial resolution. These measurements were coupled with an inverse finite element model to more precisely estimate cell tensile constitutive behavior.Both parental lines proved to be significantly (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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