Molecular recognition by Kluyveromyces of amphotericin B-loaded, galactose-tagged, poly (lactic acid) microspheres
| Autor: | Joël Coulon, Roger Bonaly, Rima Kassab, Hatem Fessi, Hélène Parrot-Lopez, Jean Menaucourt |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Antifungal Agents
Polymers Clinical Biochemistry Pharmaceutical Science macromolecular substances Microscopy Atomic Force Biochemistry Dosage form Substrate Specificity Kluyveromyces chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Amphotericin B Drug Discovery Organic chemistry Lactic Acid Molecular Biology chemistry.chemical_classification Drug Carriers Molecular Structure Organic Chemistry technology industry and agriculture Galactose Polymer Microspheres Glycolates Lactic acid Molecular Weight carbohydrates (lipids) PLGA Logistic Models chemistry Drug delivery Emulsion Molecular Medicine Liberation lipids (amino acids peptides and proteins) Drug carrier Polyglycolic Acid Nuclear chemistry |
| Zdroj: | Bioorganic & Medicinal Chemistry. 10:1767-1775 |
| ISSN: | 0968-0896 |
| Popis: | In an effort to develop a new way of drug delivery, especially for polyenic antifungal molecules, we have incorporated amphotericin B (AmB) into biodegradable galactosylated poly (L-lactic acid) (L-PLA) and poly (L-lactic-co-glycolic acid) (PLGA) microspheres. These drug carriers were prepared by solvent evaporation using an oil/water (o/w) emulsion. The ratio of galactosyl spacers with different chain lengths was 1.74-2.78%. The maximal quantity of AmB encapsulated reported to 100 mg of the galactosylated microspheres was 7.14 mg for L-PLA (encapsulation rate 45% of mole) and 6.42 mg for PLGA derivatives (encapsulation rate 81% of mole). In our yeast model, drug release depended on three factors: (i) presence of galactosylated antennae, (ii) length of galactosyl antenna and (iii) nature of the polymer. More of the AmB trapped in PLGA microspheres was released than from PLA microspheres. These novel functionalised microspheres could be required for the delivering of therapeutic agents according to their recognition to specific cells. |
| Databáze: | OpenAIRE |
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