Effects of iron supplementation in mice with hypoferremia induced by obesity
| Autor: | Alessandra Gambero, Érica Martins Ferreira Gotardo, Cintia Rabelo e Paiva Caria, Marcelo Lima Ribeiro, Caroline Candida de Oliveira, Thalita Rocha |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Iron Adipose tissue Adipokine Inflammation Intra-Abdominal Fat Ferric Compounds General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Insulin resistance Hepcidins Hepcidin Internal medicine medicine Animals Glucose homeostasis Obesity Original Research Anemia Iron-Deficiency biology business.industry Leptin medicine.disease 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis Dietary Supplements biology.protein Cytokines Resistin medicine.symptom business |
| Zdroj: | Experimental Biology and Medicine. 241:2049-2055 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/1535370216660398 |
| Popis: | Iron is an important micronutrient, but it can also act as a dangerous element by interfering with glucose homeostasis and inflammation, two features that are already disturbed in obese subjects. In this work, we study the effects of systemic iron supplementation on metabolic and inflammatory responses in mice with hypoferremia induced by obesity to better characterize whether iron worsens the parameters that are already altered after 24 weeks of a high-fat diet (HFD). Mice were maintained on a control diet or a HFD for 24 weeks and received iron-III polymaltose (50 mg/kg/every 2 days) during the last two weeks. Glucose homeostasis (basal glucose and insulin test tolerance) and systemic and visceral adipose tissue (VAT) inflammation were assessed. Iron levels were measured in serum. The Prussian blue reaction was used in isolated macrophages to detect iron deposition. Iron supplementation resulted in an increased number of VAT macrophages that were positive for Prussian blue staining as well as increased serum iron levels. Systemic hepcidin, leptin, resistin, and monocyte chemoattractant protein-1 (MCP-1) levels were not altered by iron supplementation. Local adipose tissue inflammation was also not made worse by iron supplementation because the levels of hepcidin, MCP-1, leptin, and interleukin (IL)-6 were not altered. In contrast, iron supplementation resulted in an increased production of IL-10 by adipose tissue and VAT macrophages. Leukocytosis and VAT plasminogen activator inhibitor-1 (PAI-1) level were reduced, but insulin resistance was not altered after iron supplementation. In conclusion, systemic iron supplementation in mice with hypoferremia induced by obesity did not worsen inflammatory marker or adipose tissue inflammation or the metabolic status established by obesity. Iron deposition was observed in adipose tissue, mainly in macrophages, suggesting that these cells have mechanisms that promote iron incorporation without increasing the production of inflammatory mediators. |
| Databáze: | OpenAIRE |
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