Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus Sequence Type 59 in Taiwan

Autor: Tomomi Takano, Wataru Higuchi, Hassan Zaraket, Taketo Otsuka, Tatiana Baranovich, Shymaa Enany, Kohei Saito, Hirokazu Isobe, Soshi Dohmae, Kyoko Ozaki, Misao Takano, Yasuhisa Iwao, Michiko Shibuya, Takeshi Okubo, Shizuka Yabe, Da Shi, Ivan Reva, Lee-Jene Teng, Tatsuo Yamamoto
Rok vydání: 2008
Předmět:
Adult
Staphylococcus aureus
Tetracycline
Bacterial Toxins
Molecular Sequence Data
Taiwan
Exotoxins
Microbial Sensitivity Tests
Drug resistance
Biology
medicine.disease_cause
Microbiology
Plasmid
Bacterial Proteins
Leukocidins
Mechanisms of Resistance
Drug Resistance
Multiple
Bacterial
Gene cluster
medicine
Humans
Pharmacology (medical)
skin and connective tissue diseases
Child
Pharmacology
Polymorphism
Genetic
Virulence
Sequence Analysis
DNA
respiratory system
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Virology
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Community-Acquired Infections
Multiple drug resistance
Infectious Diseases
Composite transposon
Child
Preschool
bacteria
Publisher's Correction
Methicillin Resistance
medicine.drug
Zdroj: Antimicrobial Agents and Chemotherapy. 52:837-845
ISSN: 1098-6596
0066-4804
DOI: 10.1128/aac.01001-07
Popis: Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, which often produce Panton-Valentine leucocidin (PVL), are increasingly noted worldwide. In this study, we examined 42 MRSA strains (25 PVL-positive [PVL + ] strains and 17 PVL-negative [PVL − ] strains) isolated in Taiwan for their molecular characteristics. The PVL + MRSA strains included CA-MRSA strains with multilocus sequence type (ST) 59 (major PVL + MRSA in Taiwan), its variants, and worldwide CA-MRSA ST30 strains. The PVL − MRSA strains included the pandemic Hungarian MRSA ST239 strain, the Hungarian MRSA ST239 variant, MRSA ST59 (largely hospital-acquired MRSA strains) and its variants, the pandemic New York/Japan MRSA ST5 strain (Japanese type), and the MRSA ST8 strain. The major PVL + CA-MRSA ST59 strain possessed a tetracycline resistance-conferring ( tetK positive) penicillinase plasmid and a drug resistance gene cluster (a possible composite transposon) for multidrug resistance. Moreover, it carried a novel staphylococcal cassette chromosome mec (SCC mec ) with two distinct ccrC genes ( ccrC2 - C8 ). This SCC mec (previously named SCC mec type V T ) was tentatively designated SCC mec type VII. Sequencing of the PVL genes revealed the polymorphisms, and the PVL + CA-MRSA ST59 strain possessed the ST59-specific PVL gene sequence. The data suggest that a significant amount of clonal spread is occurring in Taiwan and that the major PVL + CA-MRSA ST59 Taiwan strain exhibits unique genetic characteristics, such as a novel SCC mec type and an ST59-specific PVL gene sequence.
Databáze: OpenAIRE
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