GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A
| Autor: | Wolfgang Schreibmayer, Ruth Prassl, Stefanie Stanzer, Katja Ester, Sonja Langthaler, Nassim Ghaffari-Tabrizi-Wizsy, Simin Rezania, Andreas Prokesch, Helmut Ahammer, Dieter Platzer, Susanne Scheruebel, Brigitte Pelzmann, Klaus Zorn-Pauly, Gebhard Schratter, Trevor DeVaney, Thomas Bauernhofer, Kurt Schmidt, Astrid Gorischek, Stephan W Jahn |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell biology
Carcinogenesis Molecular biology Biophysics lcsh:Medicine Breast Neoplasms Diseases Biology Biochemistry Article GIRK1 breast cancer MCF10A MCF7 Transcriptome Extracellular matrix 03 medical and health sciences 0302 clinical medicine Breast cancer Medical research Cell Movement Neoplasms medicine Humans Mammary Glands Human skin and connective tissue diseases lcsh:Science 030304 developmental biology Cancer Regulation of gene expression 0303 health sciences Multidisciplinary Molecular medicine lcsh:R Cell migration medicine.disease 3. Good health Gene Expression Regulation Neoplastic G Protein-Coupled Inwardly-Rectifying Potassium Channels Oncology 030220 oncology & carcinogenesis Lymphatic Metastasis Cancer research MCF-7 Cells Female lcsh:Q Wound healing Biomarkers |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-18 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with the control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer. |
| Databáze: | OpenAIRE |
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