Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration
| Autor: | Keiko Nakayama, Norio Matsuki, Chihiro Ueyama, Keiichi I. Nakayama, Hiroki Akashiba, Nobuyoshi Nishiyama |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Kainic acid Blotting Western Excitotoxicity Kainate receptor Hippocampal formation medicine.disease_cause Hippocampus chemistry.chemical_compound Mice Seizures Internal medicine medicine Excitatory Amino Acid Agonists Animals Mice Knockout Kainic Acid biology Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Cell Cycle Retinoblastoma protein DNA Cell cycle Immunohistochemistry Mice Inbred C57BL Endocrinology chemistry Knockout mouse Nerve Degeneration biology.protein RNA Interference Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Neuroreport. 18(17) |
| ISSN: | 0959-4965 |
| Popis: | p27 is a cyclin-dependent kinase inhibitor which arrests cell cycle at G 1 -S phase. Using RNA interference method, we previously showed that reduction of endogenous p27 expression induces cell death through cell cycle progression in cultured cortical neurons. In this study, we investigated responses to kainate treatment using p27 knockout mice. Injection of kainic acid induced p27 downregulation and retinoblastoma protein phosphorylation in wild-type mouse hippocampus. No change was observed in hippocampal cell viability in untreated adult p27 heterozygous and homozygous mice compared with wild type (+/+). p27 homozygous mice, however, displayed enhanced seizure and hippocampal degeneration after kainic acid treatment. This study first suggests that ablation of p27 enhance kainate-induced seizure and hippocampal cell death in vivo. |
| Databáze: | OpenAIRE |
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