Evaluation of the Cytotoxic Effect of Rutin Prenanoemulsion in Lung and Colon Cancer Cell Lines
| Autor: | Nghiem Thi Thuong, Luu Hai Minh, Tran Thi Bich Dao, Duong Xuan Anh, Luu Hai Long, Phan Thi Yen, Bui Quoc Anh, Tran Thanh Hoai |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Chromatography
Materials science Article Subject Chemical structure Chemical modification 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography In vitro 0104 chemical sciences Rutin chemistry.chemical_compound chemistry PEG ratio T1-995 General Materials Science Particle size Solubility 0210 nano-technology Technology (General) |
| Zdroj: | Journal of Nanomaterials, Vol 2020 (2020) |
| ISSN: | 1687-4110 |
| DOI: | 10.1155/2020/8867669 |
| Popis: | In this work, prenanoemulsion of rutin was prepared using PEG and Tween as emulsifiers via homogenization and evaporation techniques. The particle size of rutin was investigated with high-resolution transmission electron microscopy (HR-TEM); particle size distribution and its chemical structure were analysed by nuclear magnetic resonance (NMR) and Fourier transformed infrared (FT-IR) spectroscopy. It was found that rutin in the prenanoemulsion has excellent solubility in water with the size approximately 15 nm. The chemical structure of nanorutin in prenanoemulsion was identical to that of the pure rutin. It suggested that there is no chemical modification of rutin in the prenanoemulsion. From high-performance liquid chromatography (HPLC), the amount of rutin in the prenanoemulsion was determined to be 9.27%. The cytotoxic effect of rutin in the preemulsion was investigated via in vitro tests to determine rutin’s efficacy in A549 lung cancer cell and colon cancer cell treatment. The results demonstrated that rutin in the prenanoemulsion could inhibit A549 lung cancer cells and colon cancer cells efficiently. |
| Databáze: | OpenAIRE |
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