FMLP-induced arachidonic acid release, phospholipid metabolism, and calcium mobilization in human monocytes. Regulation by cyclic AMP
| Autor: | Richard W. Godfrey, Bruce D. Jensen, Rosalie M. Manzi, Sylvia Hoffstein |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
IBMX Immunology Phospholipid chemistry.chemical_element Arachidonic Acids Calcium Phospholipase Biology Monocytes chemistry.chemical_compound Cyclic nucleotide Cytosol Internal medicine Phosphatidylcholine 1-Methyl-3-isobutylxanthine medicine Cyclic AMP Immunology and Allergy Humans Calcimycin Cells Cultured Phospholipids Arachidonic Acid Monocyte N-Formylmethionine Leucyl-Phenylalanine Endocrinology medicine.anatomical_structure chemistry Biochemistry Bucladesine Arachidonic acid Chromatography Thin Layer |
| Zdroj: | Inflammation. 12(3) |
| ISSN: | 0360-3997 |
| Popis: | Radiolabeled human peripheral blood monocytes released [3H]arachidonic acid upon challenge with the calcium ionophore A23187 (10 microM), or f-Met-Leu-Phe (FMLP, 1 microM). Chromatographic analysis of [3H]arachidonic acid labeled phospholipids showed that stimulation by FMLP reduced the amount of labeled phosphatidylcholine exclusively. Treatment of the monocytes with 10(-3) M dibutyryl cyclic AMP (d-cAMP) or 5 X 10(-4) M isobutylmethylxanthine (IBMX) substantially inhibited [3H]arachidonic acid release (30%) and depletion from labeled phosphatidylcholine (PC) in FMLP--but not calcium ionophore--stimulated cells. Using the fluorescent probe Indo-1, the FMLP-induced cytosolic calcium increase was unaffected by 10(-3) M dibutyryl cyclic AMP. The results suggest that FMLP-stimulated phospholipase activity is regulated by cyclic AMP, but not by depressing receptor-medicated increases in cytoplasmic free calcium. |
| Databáze: | OpenAIRE |
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